抗补体1q抗体(Anti-C1q)检测试剂盒

This assay has high sensitivity and excellent specificity for detection of Anti-Complement 1q Antibody (Anti-C1q).
No significant cross-reactivity or interference between Anti-Complement 1q Antibody (Anti-C1q) and analogues was observed.

Matrices listed below were spiked with certain level of Anti-Complement 1q Antibody (Anti-C1q) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Complement 1q Antibody (Anti-C1q) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	78-103	89
EDTA plasma(n=5)	78-101	82
heparin plasma(n=5)	91-98	95

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Complement 1q Antibody (Anti-C1q) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Complement 1q Antibody (Anti-C1q) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Complement 1q Antibody (Anti-C1q) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	79-97%	92-101%	79-93%	98-105%
EDTA plasma(n=5)	94-101%	97-105%	98-105%	83-94%
heparin plasma(n=5)	82-89%	85-97%	95-104%	79-103%

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37^oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37^oC;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37^oC;
6. Add 50µL Stop Solution. Read at 450nm immediately.

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Complement 1q Antibody (Anti-C1q) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Complement 1q Antibody (Anti-C1q) in the samples is then determined by comparing the O.D. of the samples to the standard curve.